Composite

Part:BBa_K1298005:Design

Designed by: CoBRA Team   Group: iGEM14_CoBRA   (2014-06-19)


PgeChia 1-2 (insert) using J04500


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 812
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 722
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 793
    Illegal BsaI site found at 1044


Design Notes

The protein code for the PgeChia 1-2 was optimized to remove illegal cut sites. It should be noted that there is no secretory tag in the biobrick, meaning that the chitinase protein is not being transported outside of the cell, but staying inside of it.


Source

BBa_K1298001 (PgeChia 1-1)

  • Phytochemistry paper of Dr. Kolosova (1)
  • NCBI (protein code) (2)
  • Bio Basic Inc. (DNA optimization)

References

1. Kolosova, N. “Cloning and characterization of chitinases from interior spruce and lodgepole pine.” 1 Oct. 2013. Feb. 2014 <http://www.ncbi.nlm.nih.gov/pubmed/24564978>

2. Kolosova, N. "Picea engelmannii x Picea glauca class I chitinase (Chia1-2) mRNA, complete cds." 30 Dec. 2013. Feb. 2014 <http://www.ncbi.nlm.nih.gov/nuccore/HM219844>*

*The sequence found at this link has been optimized to remove illegal cut sites (EcoRI, XbaI, SpeI, PstI) and therefore is not the same as the sequence found on this part page.